Product Description

The FYT-1800 Ultrasonic Cell Disruptor is a versatile instrument that utilizes the cavitation effect of ultrasound to process experimental samples. It is suitable for sample volumes ranging from 100μL to 1500 mL, with adjustable ultrasonic time and power. This device can efficiently disrupt various animal and plant tissues, cells, and bacteria, while also being capable of emulsification, separation, dispersion, extraction, and accelerating chemical reactions. It is widely applied in fields such as life sciences, materials science, and environmental protection.

Application Fields

• Bioengineering: Disruption of cells, bacteria, viruses, spores, and other cellular structures; preparation of DNA fragmentation for high-throughput sequencing and chromatin immunoprecipitation (ChIP).
• Food Engineering: Homogenization of beverages; aging acceleration of wine; dissolution enhancement and chemical reaction acceleration, such as in fat processing.
• Environmental Engineering: Homogenization of soil and rock samples; study of rock structural and physical characteristics.
• Biopharmaceuticals: Dispersion of injectable pharmaceutical substances; extraction and dispersion of herbal medicines; cracking, emulsification, homogenization, and disruption of particles like carbon nanotubes and rare-earth materials.

Selected Application Cases

Case 1: Preparation of 3D Nitrogen-Doped In₂S₃ Microspheres for Gas Sensing

Researchers from Southwest Jiaotong University, led by Yinfen Cheng, used the FYT-1800 Ultrasonic Cell Disruptor to exfoliate hydrothermally synthesized In₂S₃ microspheres into 2D single-unit-cell-thick (~3 nm) nanosheets. Simultaneously, nitrogen dopants were inserted into the lattice to create 3D nitrogen-doped In₂S₃ microspheres. These microspheres exhibited a 15% response amplitude to 10 ppm NO₂ at room temperature, demonstrating the potential of 2D doped metal sulfides for high-performance gas sensors.

Synthesis Process:

1. Dissolve 0.22 g InCl₃·4H₂O and 0.15 g thioacetamide in 40 mL deionized water.
2. Stir vigorously for 30 minutes to form a transparent solution.
3. Transfer the mixture to a 50 mL autoclave and heat at 160°C for 16 hours.
4. After cooling, subject the orange solution to probe ultrasonication (400 W, 3 hours) using the FYT-1800.
5. Centrifuge at 4000 rpm for 30 minutes to separate the dispersion.
6. Dilute 20 mL of the supernatant in ethanol to enhance material dispersity.

Case 2: Analysis of Phthalate Esters (PAEs) in Environmental and Food Samples

A team from Nankai University, led by Yaqin Ji, collected 448 samples (including food, PM10, drinking water, soil, and indoor dust) from Tianjin. The FYT-1800 was used to extract six priority PAEs with 10 mL n-hexane for exposure assessment.

Extraction Protocol:

1. Weigh 5 g of fresh food into a 25 mL glass tube.
2. Add 10 mL n-hexane and homogenize using the FYT-1800.
3. Shake the slurry for 10 minutes, then centrifuge at 3500 rpm for 5 minutes.
4. Purify the extract using Florisil solid-phase extraction (SPE).
5. Concentrate under nitrogen to a final volume of 2 mL for analysis.

Case 3: Quantitative Thiol Reactivity Profiling (QTRP) in Proteomics

Ling Fu's team at the Beijing Institute of Life Sciences developed the QTRP platform to study cysteine reactivity in HEK293T cells. The FYT-1800 (30 W, 20 kHz) was used to lyse cells, extract proteins, and shear genomic DNA to reduce viscosity.

Protocol:

1. Resuspend pelleted cells in 500μL lysis buffer.
2. Sonicate on ice with 10-second pulses (5-second intervals, 3 cycles).

Case 4: Heterologous Expression of Esterase EstP8 in E. coli

Yanhui Gong's group (Jinan University) achieved high-yield expression of esterase EstP8 in E. coli BL21(DE3) using the FYT-1800 for cell disruption. The enzyme showed metal/organic solvent tolerance and chiral resolution capability for (R)-1-phenylethanol production.

Case 5: Nanoemulsion-Based Edible Coating for Meat Preservation

Huiyun Zhang (Henan University of Science and Technology) developed a chitosan (CH)- and Schizonepeta tenuifolia essential oil (STEO)-based nanoemulsion coating. The FYT-1800 (200 W, 20 kHz) homogenized coarse emulsions into nanoemulsions (<200 nm), extending fresh pork shelf life at 4°C by 16 days.

Key Parameters:

• Pulse: 5 s on/5 s off.
• Temperature control: 25°C water bath.

Conclusion

The FYT-1800 Ultrasonic Cell Disruptor demonstrates exceptional versatility across disciplines---from nanomaterials synthesis to food safety and proteomics. Its precision in energy delivery and non-thermal processing makes it indispensable for modern laboratory workflows.