Pulsed field electrophoresis is the separation of DNA by alternately changing the electric field between pairs of pairs of DNA molecules and passing through the agarose gel pore at different migration rates. The CHEF Mapper A series is suitable for the detection and isolation of DNA molecules of 100bp-10 Mb, with a high resolution in the range, mainly used for genomic DNA isolation analysis, chromosomal DNA isolation, large fragment genomic library construction, identification and analysis, transgenic research, synthetic biology and other fields. Each CHEF Mapper A system contains the controller, electrophoresis tank, cooling device, circulating pump and accessories.

Product characteristics

1. Advanced technology: combined with CHEF, PACE's pulse field technology achieves excellent results, with straight lanes and no bends;

2 Independent control: The electrode potential composed of 24 platinum electrodes (0.5mm diameter) is independently controlled, and each electrode can be replaced separately.

3 Unique algorithms: Using a unique pulse control algorithm, the separation effect is better, and linear and circular DNA can be easily distinguished, with better separation effect for large circular DNA;

4 unique automatic calculation functions, combined with the determination of 10 main variables, help users obtain the most ideal experimental conditions;

5 Program Storage: Stores 15 complex experimental programs, each including no less than 8 program modules;

6 multi state vector transformation: up to 10 vectors/pulse period, each angle, voltage, and duration can be defined;

7 Conversion slope: Linear, concave or convex application of hyperbolic functions;

Automation: If the system is interrupted due to power failure, it can automatically record and restart electrophoresis.

9. Customizable: Users can set their own conditions, and the user manual records a large number of electrophoresis conditions under different separation spans.

10 Flexibility: The system can select voltage gradients and conversion times for specific DNA size spans.

11 large screens: 7-inch LCD display screen, easy to operate, unique software control, easy to operate.

12 Temperature detection: Dual temperature probes directly detect the temperature of the buffer solution, with an error of<± 0.5 ℃.

13 Circulation Device: Equipped with a buffer circulation system, it can accurately control and monitor the temperature of the buffer solution, ensuring constant temperature and ion balance during electrophoresis.

14. High safety: polycarbonate transparent glass safety cover, automatically cuts off power when the cover is lifted to prevent contact;

15 adjustable levels: The electrophoresis tank and gel maker are designed with adjustable support angles and adjustable levels.

16 Mold: Adopting an electrophoresis tank and an integrated integral mold structure without adhesion; The electrode holder is equipped with a platinum electrode with a diameter of 0.5mm, which is not easily deformed and has a long lifespan, ensuring stable experimental results.

17 Pause Recovery: It has pause/resume operation function, power-off time recording and intelligent recovery function.

18 polymorphism: It has the function of vector variables with multiple states, and a single pulse cycle can run up to 10 different combinations of pulse angles and running times;

19 pulse angle: The pulse angle can be freely selected between 0-360 °, allowing users to achieve effective separation of large to chromosome level and small to plasmid DNA on the same system.

There are two types of pulse time gradients: linear and nonlinear (convex and concave). Nonlinear gradients can provide a wider dynamic range for separation, allowing users to accurately determine the size of separation segments.

21 Real time monitoring: Simultaneously display set parameters and working conditions, can be matched with real-time monitoring upper computer software.

22 Secondary pulse function: secondary pulse can accelerate the release of DNA from agarose gel, which is conducive to the separation of very large DNA fragments and can improve the resolution.

23 compatible with PulseNet China: able to connect with the national pathogen monitoring network and PulseNet China monitoring network, and distinguish fragments with similar molecular weights.

technical specifications

● Voltage gradient: 0.5V/cm-9.6V/cm, increment of 0.1V/cm;

● Maximum current: 0.5A;

● Maximum voltage: 350V;

● Pulse angle: 0-360 °, increments of 0.1 °;

● Time gradient: linear and nonlinear;  

● Conversion time: 50ms to 18h;

● Maximum running time: 999h;

● Number of electrodes: 24, independently controlled;

● Having polymorphic function;

● Temperature range: 0 ℃~50 ℃, detection error<± 0.5 ℃.

● Up to 2.4L buffer solution

 

Comparison Table of Functions of Different Models of Pulse Field Electrophoresis System

 

Configuration list

 

The CHEF systems that incorporate different technologies for optimal resolution in various size ranges, such as PACE, FIGE, PFGE and AFIGE are offered.